Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity.

Published

Journal Article

Recent studies of human immunodeficiency virus type 1 (HIV-1) infection in humans and of simian immunodeficiency virus (SIV) in rhesus monkeys have shown that resolution of the acute viral infection and control of the subsequent persistent infection are mediated by the antiviral cellular immune response. We comparatively assessed several vaccine vector delivery systems-three formulations of a plasmid DNA vector, the modified vaccinia Ankara (MVA) virus, and a replication incompetent adenovirus type 5 (Ad5) vector-expressing the SIV gag protein for their ability to elicit such immune responses in monkeys. The vaccines were tested either as a single modality or in combined modality regimens. Here we show that the most effective responses were elicited by a replication-incompetent Ad5 vector, used either alone or as a booster inoculation after priming with a DNA vector. After challenge with a pathogenic HIV-SIV hybrid virus (SHIV), the animals immunized with Ad5 vector exhibited the most pronounced attenuation of the virus infection. The replication-defective adenovirus is a promising vaccine vector for development of an HIV-1 vaccine.

Full Text

Duke Authors

Cited Authors

  • Shiver, JW; Fu, T-M; Chen, L; Casimiro, DR; Davies, M-E; Evans, RK; Zhang, Z-Q; Simon, AJ; Trigona, WL; Dubey, SA; Huang, L; Harris, VA; Long, RS; Liang, X; Handt, L; Schleif, WA; Zhu, L; Freed, DC; Persaud, NV; Guan, L; Punt, KS; Tang, A; Chen, M; Wilson, KA; Collins, KB; Heidecker, GJ; Fernandez, VR; Perry, HC; Joyce, JG; Grimm, KM; Cook, JC; Keller, PM; Kresock, DS; Mach, H; Troutman, RD; Isopi, LA; Williams, DM; Xu, Z; Bohannon, KE; Volkin, DB; Montefiori, DC; Miura, A; Krivulka, GR; Lifton, MA; Kuroda, MJ; Schmitz, JE; Letvin, NL; Caulfield, MJ; Bett, AJ; Youil, R; Kaslow, DC; Emini, EA

Published Date

  • January 2002

Published In

Volume / Issue

  • 415 / 6869

Start / End Page

  • 331 - 335

PubMed ID

  • 11797011

Pubmed Central ID

  • 11797011

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/415331a

Language

  • eng