Immunogenicity and protective efficacy of oligomeric human immunodeficiency virus type 1 gp140.
Journal Article (Journal Article)
The biologically active form of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) glycoprotein is oligomeric. We previously described a soluble HIV-1 IIIB Env protein, gp140, with a stable oligomeric structure composed of uncleaved gp120 linked to the ectodomain of gp41 (P. L. Earl, C. C. Broder, D. Long, S. A. Lee, J. Peterson, S. Chakrabarti, R. W. Doms, and B. Moss, J. Virol. 68:3015-3026, 1994). Here we compared the antibody responses of rabbits to gp120 and gp140 that had been produced and purified in an identical manner. The gp140 antisera exhibited enhanced cross-reactivity with heterologous Env proteins as well as greater neutralization of HIV-1 compared to the gp120 antisera. To examine both immunogenicity and protective efficacy, we immunized rhesus macaques with oligomeric gp140. Strong neutralizing antibodies against a homologous virus and modest neutralization of heterologous laboratory-adapted isolates were elicited. No neutralization of primary isolates was observed. However, a substantial fraction of the neutralizing activity could not be blocked by a V3 loop peptide. After intravenous challenge with simian-HIV virus SHIV-HXB2, three of the four vaccinated macaques exhibited no evidence of virus replication.
Full Text
Duke Authors
Cited Authors
- Earl, PL; Sugiura, W; Montefiori, DC; Broder, CC; Lee, SA; Wild, C; Lifson, J; Moss, B
Published Date
- January 2001
Published In
Volume / Issue
- 75 / 2
Start / End Page
- 645 - 653
PubMed ID
- 11134278
Pubmed Central ID
- PMC113961
International Standard Serial Number (ISSN)
- 0022-538X
Digital Object Identifier (DOI)
- 10.1128/JVI.75.2.645-653.2001
Language
- eng
Conference Location
- United States