Vaccination of macaques against pathogenic simian immunodeficiency virus with Venezuelan equine encephalitis virus replicon particles.

Published

Journal Article

Vaccine vectors derived from Venezuelan equine encephalitis virus (VEE) that expressed simian immunodeficiency virus (SIV) immunogens were tested in rhesus macaques as part of the effort to design a safe and effective vaccine for human immunodeficiency virus. Immunization with VEE replicon particles induced both humoral and cellular immune responses. Four of four vaccinated animals were protected against disease for at least 16 months following intravenous challenge with a pathogenic SIV swarm, while two of four controls required euthanasia at 10 and 11 weeks. Vaccination reduced the mean peak viral load 100-fold. The plasma viral load was reduced to below the limit of detection (1,500 genome copies/ml) in one vaccinated animal between 6 and 16 weeks postchallenge and in another from week 6 through the last sampling time (40 weeks postchallenge). The extent of reduction in challenge virus replication was directly correlated with the strength of the immune response induced by the vectors, which suggests that vaccination was effective.

Full Text

Duke Authors

Cited Authors

  • Davis, NL; Caley, IJ; Brown, KW; Betts, MR; Irlbeck, DM; McGrath, KM; Connell, MJ; Montefiori, DC; Frelinger, JA; Swanstrom, R; Johnson, PR; Johnston, RE

Published Date

  • January 2000

Published In

Volume / Issue

  • 74 / 1

Start / End Page

  • 371 - 378

PubMed ID

  • 10590126

Pubmed Central ID

  • 10590126

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.74.1.371-378.2000

Language

  • eng

Conference Location

  • United States