Neutralizing antibody responses in Africa green monkeys naturally infected with simian immunodeficiency virus (SIVagm).

Published

Journal Article

This study assessed the magnitude and cross-reactivity of the neutralizing antibody response generated by natural SIV infection in wild-caught African green monkeys. Neutralizing antibodies of variable potency, sometimes exceeding a titer of 1:1,000, were detected in 20 of 20 SIV-seropositive African green monkeys in Kenya. Detection of those neutralizing antibodies was dependent on the strain of virus and the cells used for assay, where the most sensitive detection was made with SIVagm1532 in Sup T1 cells. Potent neutralization of SIVagm1532 was seen with contemporaneous autologous serum. Potent neutralization was also detected with laboratory-passaged SIVmac251 and SIVsmB670, but not with SIVsmE660 and two additional strains of SIVagm. Serum samples from rhesus macaques (Macaca mulatta) experimentally infected with either SIVmac251 or SIVsmE660 were capable of low-level neutralization of SIVagm. These results indicate that natural infection with SIV can generate strain-specific neutralizing antibodies in African green monkeys. They also indicate that some neutralization determinants of SIVagm are partially shared with SIV strains that arose in sooty mangabys and were subsequently transmitted to rhesus macaques.

Full Text

Duke Authors

Cited Authors

  • Gicheru, MM; Otsyula, M; Spearman, P; Graham, BS; Miller, CJ; Robinson, HL; Haigwood, NL; Montefiori, DC

Published Date

  • June 1999

Published In

Volume / Issue

  • 28 / 3

Start / End Page

  • 97 - 104

PubMed ID

  • 10475110

Pubmed Central ID

  • 10475110

International Standard Serial Number (ISSN)

  • 0047-2565

Digital Object Identifier (DOI)

  • 10.1111/j.1600-0684.1999.tb00257.x

Language

  • eng

Conference Location

  • Denmark