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Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation.

Publication ,  Journal Article
Zou, Z; Zhang, W; Young, D; Gleave, MG; Rennie, P; Connell, T; Connelly, R; Moul, J; Srivastava, S; Sesterhenn, I
Published in: Clin Cancer Res
May 2002

PURPOSE: Expression of tumor suppressor gene, MASPIN, is associated with inhibition of tumor cell invasion and metastasis. Loss of or decreased expression of Maspin is found frequently in breast and prostate cancer cells. The objective of this study is to investigate Maspin expression in prostate tumor specimens and explore the mechanisms of hormonal regulation of Maspin expression in prostate tumors. EXPERIMENTAL DESIGN: Immunohistochemical staining of Maspin expression was performed on surgical whole-mounted prostate specimens. The expression of Maspin was scored on individual tumors. Correlation of Maspin expression with clinicopathological features was analyzed for statistical significance. Androgen ablation-induced Maspin expression was analyzed by Maspin promoter luciferase reporter assay and quantitative reverse transcription-PCR analysis of endogenous Maspin expression in LNCaP cells in vitro and in animal model. RESULTS: Comprehensive evaluation of Maspin expression profile in multiple tumor foci from whole mounted prostate specimens of prostate cancer patients revealed absence of Maspin expression in a significant fraction (63%). However, Maspin expression is significantly higher in tumor specimens (92%) of patients treated with neoadjuvant androgen ablation therapy before radical prostatectomy. LNCaP cells cultured in androgen-depleted medium show induction of Maspin promoter activity in a promoter luciferase reporter assay. In addition, Maspin expression is increased after castration in LNCaP prostate cancer cells derived tumors in nude mice. CONCLUSIONS: Maspin expression is frequently absent in primary prostate cancers. Up-regulation of MASPIN in response to androgen ablation strongly suggests a physiological role of Maspin in growth inhibition and/or apoptosis of prostate cancer cells during androgen ablation.

Duke Scholars

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

May 2002

Volume

8

Issue

5

Start / End Page

1172 / 1177

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Time Factors
  • Serpins
  • Recombinant Fusion Proteins
  • RNA, Neoplasm
  • Proteins
  • Protein Biosynthesis
  • Prostatic Neoplasms
  • Promoter Regions, Genetic
 

Citation

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Zou, Z., Zhang, W., Young, D., Gleave, M. G., Rennie, P., Connell, T., … Sesterhenn, I. (2002). Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation. Clin Cancer Res, 8(5), 1172–1177.
Zou, Zhiqiang, Wei Zhang, Denise Young, Martin G. Gleave, Paul Rennie, Theresa Connell, Roger Connelly, Judd Moul, Shiv Srivastava, and Isabell Sesterhenn. “Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation.Clin Cancer Res 8, no. 5 (May 2002): 1172–77.
Zou Z, Zhang W, Young D, Gleave MG, Rennie P, Connell T, et al. Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation. Clin Cancer Res. 2002 May;8(5):1172–7.
Zou, Zhiqiang, et al. “Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation.Clin Cancer Res, vol. 8, no. 5, May 2002, pp. 1172–77.
Zou Z, Zhang W, Young D, Gleave MG, Rennie P, Connell T, Connelly R, Moul J, Srivastava S, Sesterhenn I. Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation. Clin Cancer Res. 2002 May;8(5):1172–1177.

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

May 2002

Volume

8

Issue

5

Start / End Page

1172 / 1177

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Time Factors
  • Serpins
  • Recombinant Fusion Proteins
  • RNA, Neoplasm
  • Proteins
  • Protein Biosynthesis
  • Prostatic Neoplasms
  • Promoter Regions, Genetic