The Rb-related p107 protein can suppress E2F function independently of binding to cyclin A/cdk2.


Journal Article

The interaction of the retinoblastoma susceptibility gene product (Rb)-related p107 protein with the E2F transcription factor in S-phase cells facilitates the formation of a multicomponent complex also containing cyclin A and the p33cdk2 kinase. We have created a series of p107 mutants to assess the ability of p107 to inhibit E2F function and the role of the cyclin A/cdk2 complex in this process. We find that p107 mutants that do not bind to E2F also fail to repress E2F-dependent transcription. Moreover, we find that the ability of p107 to suppress E2F-dependent transcription is not dependent on the ability of p107 to associate with cyclin A/cdk2. Finally, an analysis of the ability of the p107 mutant proteins to suppress cell growth suggests that both E2F-dependent and E2F-independent events correlate with this activity.

Full Text

Cited Authors

  • Smith, EJ; Nevins, JR

Published Date

  • January 1995

Published In

Volume / Issue

  • 15 / 1

Start / End Page

  • 338 - 344

PubMed ID

  • 7799940

Pubmed Central ID

  • 7799940

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.15.1.338


  • eng