Distinct DNA targets for trans-activation by HTLV-1 tax and adenovirus E1A.

Published

Journal Article

The HTLV-1 LTR is trans-activated by both the HTLV-1 tax (p40x) and adenovirus E1A gene products. Previous experiments have localized tax-responsive cis-elements to three 21-bp repeat units within the promoter, as well as a fourth region located between the middle and proximal repeats. A sequence TGACG, resembling the ATF/CREB recognition element, is located at the center of each of these repeat units. Mutation of this ATF/CREB site in the 21-bp repeats impairs both tax and E1A-dependent trans-activation. However, assays of a variety of promoter mutants demonstrate that sequences required for E1A and tax induction differ, suggesting that these two viral trans-activators target different factors. In addition, although the adenovirus E4 promoter also contains three ATF/CREB sites involved in E1A activation, tax does not activate this promoter. Finally, we also find that the TATAA element of the HTLV-1 LTR contributes to E1A-dependent activation but not tax activation. We concluded that although both trans-activators exhibit similarities in their activation properties, the targets for activation must differ.

Full Text

Cited Authors

  • Nicholas, J; Nevins, JR

Published Date

  • May 1991

Published In

Volume / Issue

  • 182 / 1

Start / End Page

  • 156 - 167

PubMed ID

  • 1827222

Pubmed Central ID

  • 1827222

Electronic International Standard Serial Number (EISSN)

  • 1096-0341

International Standard Serial Number (ISSN)

  • 2514-4138

Digital Object Identifier (DOI)

  • 10.1016/0042-6822(91)90659-y

Language

  • eng