Glucose-stimulated insulin secretion in cell lines
Because islet β-cells are expensive and difficult to isolate, islet transplantation may have limited applicability for insulin replacement in insulin-dependent diabetes mellitus. Knowledge of glucose sensing guides the development of clonal cell lines that behave Like β-cells. High-K(m) glucose transport and glucose phosphorylation both contribute to glucose-stimulated insulin secretion in clonal cells and β-cells. Introduction of these and other key molecular components of the β-cell into clonal lines may eventually result in glucose-responsive, insulin-secreting cell implants for human use.