Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene.

Journal Article (Journal Article)

The Mtl gene from Cryptococcus neoformans, which confers the ability of Saccharomyces cerevisiae Sc4l YJO to grow on mannitol with substantial NAD-dependent mannitol dehydrogenase activity, was identified. Purifications and characterizations of this enzyme show that it is found in polyploid strain BB1, and the peptide sequence of the enzyme helped identify the saccharomyces gene encoding this mannitol dehydrogenase activity. On the other hand, the Mtl gene of C. neoformans encodes a 346-amino-acid protein which is not mannitol dehydrogenase but a regulatory element which is active in a heterologous fungus.

Full Text

Duke Authors

Cited Authors

  • Perfect, JR; Rude, TH; Wong, B; Flynn, T; Chaturvedi, V; Niehaus, W

Published Date

  • September 1996

Published In

Volume / Issue

  • 178 / 17

Start / End Page

  • 5257 - 5262

PubMed ID

  • 8752346

Pubmed Central ID

  • PMC178325

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.178.17.5257-5262.1996


  • eng

Conference Location

  • United States