Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene.
Journal Article (Journal Article)
The Mtl gene from Cryptococcus neoformans, which confers the ability of Saccharomyces cerevisiae Sc4l YJO to grow on mannitol with substantial NAD-dependent mannitol dehydrogenase activity, was identified. Purifications and characterizations of this enzyme show that it is found in polyploid strain BB1, and the peptide sequence of the enzyme helped identify the saccharomyces gene encoding this mannitol dehydrogenase activity. On the other hand, the Mtl gene of C. neoformans encodes a 346-amino-acid protein which is not mannitol dehydrogenase but a regulatory element which is active in a heterologous fungus.
Full Text
Duke Authors
Cited Authors
- Perfect, JR; Rude, TH; Wong, B; Flynn, T; Chaturvedi, V; Niehaus, W
Published Date
- September 1996
Published In
Volume / Issue
- 178 / 17
Start / End Page
- 5257 - 5262
PubMed ID
- 8752346
Pubmed Central ID
- PMC178325
International Standard Serial Number (ISSN)
- 0021-9193
Digital Object Identifier (DOI)
- 10.1128/jb.178.17.5257-5262.1996
Language
- eng
Conference Location
- United States