Genetic mapping of Ty elements in Saccharomyces cerevisiae.

Journal Article (Journal Article)

We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.

Full Text

Duke Authors

Cited Authors

  • Klein, HL; Petes, TD

Published Date

  • February 1, 1984

Published In

Volume / Issue

  • 4 / 2

Start / End Page

  • 329 - 339

PubMed ID

  • 6366520

Pubmed Central ID

  • PMC368700

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.4.2.329-339.1984

Language

  • eng

Conference Location

  • United States