Is there left-handed DNA at the ends of yeast chromosomes?

Published

Journal Article

Tracts of the alternating copolymer poly(dGdT . dCdA) have been observed in a variety of eukaryotes. Such tracts are of particular interest since homopolymers of this sequence can exist in vitro as left-handed Z form DNA. We have found that the yeast Saccharomyces cerevisiae contains at least 30 poly(GT) tracts at dispersed genomic locations. We show here that one subset of these tracts is located at the ends (telomeres) of the yeast chromosome. In addition, we show that poly(dGdT . dCdA) tracts are added to the ends of the extrachromosomal ribosomal DNA molecules of Tetrahymena when cloned in yeast. These data represent the first reported association between a homopolymeric sequence and a chromosome structure.

Full Text

Duke Authors

Cited Authors

  • Walmsley, RM; Szostak, JW; Petes, TD

Published Date

  • March 1983

Published In

Volume / Issue

  • 302 / 5903

Start / End Page

  • 84 - 86

PubMed ID

  • 6338397

Pubmed Central ID

  • 6338397

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/302084a0

Language

  • eng