Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity.
Journal Article (Journal Article)
As a model system for studying the properties of mitotic recombination in the yeast Saccharomyces cerevisiae, we have examined recombination between a recombinant plasmid (introduced into the S. cerevisiae cell by transformation) and homologous chromosomal loci. The recombinant plasmids used in these experiments contained S. cerevisiae rRNA genes. We found that the frequency of integrative recombination is sensitive to small amounts of sequence heterogeneity. In addition, the frequency and specificity of these recombination events are affected by the lengths of the interacting homologous DNA sequences.
Full Text
Duke Authors
Cited Authors
- Smolik-Utlaut, S; Petes, TD
Published Date
- July 1, 1983
Published In
Volume / Issue
- 3 / 7
Start / End Page
- 1204 - 1211
PubMed ID
- 6350848
Pubmed Central ID
- 6350848
International Standard Serial Number (ISSN)
- 0270-7306
Digital Object Identifier (DOI)
- 10.1128/mcb.3.7.1204
Language
- eng
Conference Location
- United States