Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity.

Journal Article

As a model system for studying the properties of mitotic recombination in the yeast Saccharomyces cerevisiae, we have examined recombination between a recombinant plasmid (introduced into the S. cerevisiae cell by transformation) and homologous chromosomal loci. The recombinant plasmids used in these experiments contained S. cerevisiae rRNA genes. We found that the frequency of integrative recombination is sensitive to small amounts of sequence heterogeneity. In addition, the frequency and specificity of these recombination events are affected by the lengths of the interacting homologous DNA sequences.

Full Text

Duke Authors

Cited Authors

  • Smolik-Utlaut, S; Petes, TD

Published Date

  • July 1983

Published In

Volume / Issue

  • 3 / 7

Start / End Page

  • 1204 - 1211

PubMed ID

  • 6350848

International Standard Serial Number (ISSN)

  • 0270-7306

Language

  • eng

Conference Location

  • United States