Isolation and analysis of recombinant DNA molecules containing yeast DNA.

Journal Article

2500 recombinant plasmids containing insertions of yeast nuclear DNA have been cloned in Escherichia coli. It can be calculated that about 85% of the yeast genome is represented in this collection. The clones have been characterized by hybridization to purified RNA species. Of the 2000 clones examined, 75 contain insertions of yeast ribosomal DNA, 201 contain insertions of yeast tRNA genes, and 26 contain DNA sequences that are complementary to abundant mRNA species.

Full Text

Duke Authors

Cited Authors

  • Petes, TD; Broach, JR; Wensink, PC; Hereford, LM; Fink, GR; Botstein, D

Published Date

  • September 1, 1978

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 37 - 49

PubMed ID

  • 365691

International Standard Serial Number (ISSN)

  • 0378-1119

Language

  • eng

Conference Location

  • Netherlands