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Isolation and analysis of recombinant DNA molecules containing yeast DNA.

Publication ,  Journal Article
Petes, TD; Broach, JR; Wensink, PC; Hereford, LM; Fink, GR; Botstein, D
Published in: Gene
September 1978

2500 recombinant plasmids containing insertions of yeast nuclear DNA have been cloned in Escherichia coli. It can be calculated that about 85% of the yeast genome is represented in this collection. The clones have been characterized by hybridization to purified RNA species. Of the 2000 clones examined, 75 contain insertions of yeast ribosomal DNA, 201 contain insertions of yeast tRNA genes, and 26 contain DNA sequences that are complementary to abundant mRNA species.

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Published In

Gene

DOI

ISSN

0378-1119

Publication Date

September 1978

Volume

4

Issue

1

Start / End Page

37 / 49

Location

Netherlands

Related Subject Headings

  • Saccharomyces cerevisiae
  • RNA, Transfer
  • RNA, Ribosomal
  • RNA, Messenger
  • RNA
  • Plasmids
  • Nucleic Acid Hybridization
  • Escherichia coli
  • Developmental Biology
  • DNA, Recombinant
 

Citation

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Petes, T. D., Broach, J. R., Wensink, P. C., Hereford, L. M., Fink, G. R., & Botstein, D. (1978). Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene, 4(1), 37–49. https://doi.org/10.1016/0378-1119(78)90013-6
Petes, T. D., J. R. Broach, P. C. Wensink, L. M. Hereford, G. R. Fink, and D. Botstein. “Isolation and analysis of recombinant DNA molecules containing yeast DNA.Gene 4, no. 1 (September 1978): 37–49. https://doi.org/10.1016/0378-1119(78)90013-6.
Petes TD, Broach JR, Wensink PC, Hereford LM, Fink GR, Botstein D. Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene. 1978 Sep;4(1):37–49.
Petes, T. D., et al. “Isolation and analysis of recombinant DNA molecules containing yeast DNA.Gene, vol. 4, no. 1, Sept. 1978, pp. 37–49. Pubmed, doi:10.1016/0378-1119(78)90013-6.
Petes TD, Broach JR, Wensink PC, Hereford LM, Fink GR, Botstein D. Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene. 1978 Sep;4(1):37–49.
Journal cover image

Published In

Gene

DOI

ISSN

0378-1119

Publication Date

September 1978

Volume

4

Issue

1

Start / End Page

37 / 49

Location

Netherlands

Related Subject Headings

  • Saccharomyces cerevisiae
  • RNA, Transfer
  • RNA, Ribosomal
  • RNA, Messenger
  • RNA
  • Plasmids
  • Nucleic Acid Hybridization
  • Escherichia coli
  • Developmental Biology
  • DNA, Recombinant