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Characterization of two types of yeast ribosomal DNA genes.

Publication ,  Journal Article
Petes, TD; Hereford, LM; Skryabin, KG
Published in: J Bacteriol
April 1978

The intragenic organization of ribosomal DNA from a diploid strain of Saccharomyces cerevisiae was analyzed by using recombinant DNA molecules constructed in vitro. Restriction analysis of the yeast ribosomal DNA with the EcoRI restriction enzyme indicated that eight restriction fragments were present in the ribosomal DNA of this strain: X' (1.87 X 10(6) daltons), A (1.77 X 10(6) daltons), B (1.48 X 10(6) daltons), C (1.22 X 10(6) daltons), D (0.39 X 10(6) daltons), E (0.36 X 10(6) daltons), F (0.22 X 10(6) daltons), and G (0.17 X 10(6) daltons). These fragments were distributed between two different types of ribosomal DNA genes, which had the restriction maps: (formula: see text) in which the underlined region shows the repeating unit. The diploid yeast strain contained approximately equal amounts of each of these two types of genes. The analysis of the recombinant DNA molecules also indicated that the yeast ribosomal genes are homogeneous and extensively clustered.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

April 1978

Volume

134

Issue

1

Start / End Page

295 / 305

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • RNA, Ribosomal
  • Plasmids
  • Microbiology
  • Genes
  • DNA, Recombinant
  • DNA Restriction Enzymes
  • DNA
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

Citation

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Petes, T. D., Hereford, L. M., & Skryabin, K. G. (1978). Characterization of two types of yeast ribosomal DNA genes. J Bacteriol, 134(1), 295–305. https://doi.org/10.1128/jb.134.1.295-305.1978
Petes, T. D., L. M. Hereford, and K. G. Skryabin. “Characterization of two types of yeast ribosomal DNA genes.J Bacteriol 134, no. 1 (April 1978): 295–305. https://doi.org/10.1128/jb.134.1.295-305.1978.
Petes TD, Hereford LM, Skryabin KG. Characterization of two types of yeast ribosomal DNA genes. J Bacteriol. 1978 Apr;134(1):295–305.
Petes, T. D., et al. “Characterization of two types of yeast ribosomal DNA genes.J Bacteriol, vol. 134, no. 1, Apr. 1978, pp. 295–305. Pubmed, doi:10.1128/jb.134.1.295-305.1978.
Petes TD, Hereford LM, Skryabin KG. Characterization of two types of yeast ribosomal DNA genes. J Bacteriol. 1978 Apr;134(1):295–305.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

April 1978

Volume

134

Issue

1

Start / End Page

295 / 305

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • RNA, Ribosomal
  • Plasmids
  • Microbiology
  • Genes
  • DNA, Recombinant
  • DNA Restriction Enzymes
  • DNA
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences