Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat.
Journal Article (Journal Article)
Reflectance spectrophotometry through the skull was used to investigate carbon monoxide (CO) binding by tissue hemoproteins in the brains of barbiturate-anesthetized Sprague-Dawley rats. After splenectomy and extensive perfluorotributylamine exchange transfusion, steady-state spectral scans were obtained in Soret and visible wave-length regions during O2 ventilation, during subsequent exposure to O2-enriched gases containing 1, 3, or 5% CO, and finally after N2 anoxia. These CO exposures were well-tolerated and electroencephalograph (EEG) activity continued to be present. Initial difference spectra were influenced by CO binding to residual hemoglobin, but spectral evidence of CO-mediated b-type cytochrome reduction was obtained in the visible region as CO concentration was increased to 3 or 5%. This was associated with Soret spectra compatible with formation of the reduced cytochrome a3-CO complex. Reduction of cytochrome a at 605 nm and cytochrome c + c1 at 550 nm was absent. These findings may indicate respiratory chain branching through b cytochromes, either to a separate a3-like oxidase independent of the classical cytochrome aa3 or to an unidentified alternative CO-sensitive oxidase.
Full Text
Duke Authors
Cited Authors
- Piantadosi, CA; Sylvia, AL; Saltzman, HA; Jöbsis-Vandervliet, FF
Published Date
- February 1985
Published In
Volume / Issue
- 58 / 2
Start / End Page
- 665 - 672
PubMed ID
- 2984161
Pubmed Central ID
- 2984161
International Standard Serial Number (ISSN)
- 8750-7587
Digital Object Identifier (DOI)
- 10.1152/jappl.1985.58.2.665
Language
- eng
Conference Location
- United States