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Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat.

Publication ,  Journal Article
Piantadosi, CA; Sylvia, AL; Saltzman, HA; Jöbsis-Vandervliet, FF
Published in: J Appl Physiol (1985)
February 1985

Reflectance spectrophotometry through the skull was used to investigate carbon monoxide (CO) binding by tissue hemoproteins in the brains of barbiturate-anesthetized Sprague-Dawley rats. After splenectomy and extensive perfluorotributylamine exchange transfusion, steady-state spectral scans were obtained in Soret and visible wave-length regions during O2 ventilation, during subsequent exposure to O2-enriched gases containing 1, 3, or 5% CO, and finally after N2 anoxia. These CO exposures were well-tolerated and electroencephalograph (EEG) activity continued to be present. Initial difference spectra were influenced by CO binding to residual hemoglobin, but spectral evidence of CO-mediated b-type cytochrome reduction was obtained in the visible region as CO concentration was increased to 3 or 5%. This was associated with Soret spectra compatible with formation of the reduced cytochrome a3-CO complex. Reduction of cytochrome a at 605 nm and cytochrome c + c1 at 550 nm was absent. These findings may indicate respiratory chain branching through b cytochromes, either to a separate a3-like oxidase independent of the classical cytochrome aa3 or to an unidentified alternative CO-sensitive oxidase.

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Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

February 1985

Volume

58

Issue

2

Start / End Page

665 / 672

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Rats, Inbred Strains
  • Rats
  • Physiology
  • Perfusion
  • Oxidation-Reduction
  • Male
  • Hemoglobins
  • Fluorocarbons
  • Electron Transport Complex IV
 

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Piantadosi, C. A., Sylvia, A. L., Saltzman, H. A., & Jöbsis-Vandervliet, F. F. (1985). Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat. J Appl Physiol (1985), 58(2), 665–672. https://doi.org/10.1152/jappl.1985.58.2.665
Piantadosi, C. A., A. L. Sylvia, H. A. Saltzman, and F. F. Jöbsis-Vandervliet. “Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat.J Appl Physiol (1985) 58, no. 2 (February 1985): 665–72. https://doi.org/10.1152/jappl.1985.58.2.665.
Piantadosi CA, Sylvia AL, Saltzman HA, Jöbsis-Vandervliet FF. Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat. J Appl Physiol (1985). 1985 Feb;58(2):665–72.
Piantadosi, C. A., et al. “Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat.J Appl Physiol (1985), vol. 58, no. 2, Feb. 1985, pp. 665–72. Pubmed, doi:10.1152/jappl.1985.58.2.665.
Piantadosi CA, Sylvia AL, Saltzman HA, Jöbsis-Vandervliet FF. Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat. J Appl Physiol (1985). 1985 Feb;58(2):665–672.

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

February 1985

Volume

58

Issue

2

Start / End Page

665 / 672

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Rats, Inbred Strains
  • Rats
  • Physiology
  • Perfusion
  • Oxidation-Reduction
  • Male
  • Hemoglobins
  • Fluorocarbons
  • Electron Transport Complex IV