Cyanide-induced cytochrome a,a3 oxidation-reduction responses in rat brain in vivo.

Journal Article (Journal Article)

The sensitivity of the brain to cyanide-induced histotoxic hypoxia and the protective effects of known cyanide antagonists, have been assessed in vivo by reflectance spectrophotometry. Cyanide-related changes in cytochrome a,a3 (cytochrome c oxidase) oxidation-reduction (redox) state, tissue hemoglobin saturation, and local blood volume were continuously monitored in cerebral cortex of rats. Noncumulative, dose-dependent inhibition of the in situ mitochondrial respiratory chain was evaluated directly by measuring increases in reduction levels of the terminal oxidase. These transient cytochrome a,a3 reductions were accompanied by increases in regional cerebral hemoglobin saturation and blood volume. Cytochrome redox responses were not altered either in magnitude or kinetics by hyperoxia; however, the cyanide-cytochrome dose-response curve was greatly shifted to the right by pretreatment with sodium nitrite, and the recovery rate of cytochrome a,a3 from cyanide-induced reduction was enhanced fourfold by pretreatment with sodium thiosulfate.

Full Text

Duke Authors

Cited Authors

  • Piantadosi, CA; Sylvia, AL; Jöbsis, FF

Published Date

  • October 1, 1983

Published In

Volume / Issue

  • 72 / 4

Start / End Page

  • 1224 - 1233

PubMed ID

  • 6313756

Pubmed Central ID

  • PMC370406

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/JCI111078


  • eng

Conference Location

  • United States