A new method for the cytological analysis of autoantibody specificities using whole-mount, surface-spread meiotic nuclei.

Journal Article (Journal Article)

A new method for the cytological analysis of antinuclear antibody binding offers several advantages over conventional techniques. Nuclei in meiosis, prepared by surface-spreading spermatocytes, provide a detailed examination of the constituents of the nucleus--euchromatin, heterochromatin, sex chromatin, nucleoli, centromeres, and dense patches that seem related to RNA metabolism--and each of these structures can be seen to change in morphology and antibody labeling during the course of meiotic prophase. Results using sera from humans and mice with autoimmune disease, and using several mouse monoclonal antibodies, demonstrate the potential of this method for clinical and research applications, both for more common antibody types and for those that bind epitopes which are unique to germ cells.

Full Text

Duke Authors

Cited Authors

  • Dresser, M; Pisetsky, D; Warren, R; McCarty, G; Moses, M

Published Date

  • November 23, 1987

Published In

Volume / Issue

  • 104 / 1-2

Start / End Page

  • 111 - 121

PubMed ID

  • 3316391

International Standard Serial Number (ISSN)

  • 0022-1759

Digital Object Identifier (DOI)

  • 10.1016/0022-1759(87)90494-7


  • eng

Conference Location

  • Netherlands