Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor.
Journal Article (Journal Article)
A dual channel, evanescent fluoroimmunoassay format is used to detect femtomolar analyte concentrations (i.e. less than 1 part per trillion [w/w]) on an etched channel siliconoxynitride thin film integrated optical waveguide. Two assays are used to demonstrate the dose-response behaviour of the sensor: (1) a direct assay of a fluorescently-labeled protein ligand binding to an immobilized protein receptor, and (2) an indirect sandwich assay of a non-fluorescent protein ligand binding to an immobilized protein receptor, as detected by the binding of a fluorescently-labeled secondary receptor protein. A red-emitting cyanine dye (Cy-5), which minimized background fluorescence and scatter losses of the waveguide, was used in both assays. To our knowledge, this is the first report of femtomolar sensitivity in an immunosensing instrument.
Full Text
Duke Authors
Cited Authors
- Plowman, TE; Reichert, WM; Peters, CR; Wang, HK; Christensen, DA; Herron, JN
Published Date
- January 1996
Published In
Volume / Issue
- 11 / 1-2
Start / End Page
- 149 - 160
PubMed ID
- 8600915
Electronic International Standard Serial Number (EISSN)
- 1873-4235
International Standard Serial Number (ISSN)
- 0956-5663
Digital Object Identifier (DOI)
- 10.1016/0956-5663(96)83722-5
Language
- eng