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Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor.

Publication ,  Journal Article
Plowman, TE; Reichert, WM; Peters, CR; Wang, HK; Christensen, DA; Herron, JN
Published in: Biosensors & bioelectronics
January 1996

A dual channel, evanescent fluoroimmunoassay format is used to detect femtomolar analyte concentrations (i.e. less than 1 part per trillion [w/w]) on an etched channel siliconoxynitride thin film integrated optical waveguide. Two assays are used to demonstrate the dose-response behaviour of the sensor: (1) a direct assay of a fluorescently-labeled protein ligand binding to an immobilized protein receptor, and (2) an indirect sandwich assay of a non-fluorescent protein ligand binding to an immobilized protein receptor, as detected by the binding of a fluorescently-labeled secondary receptor protein. A red-emitting cyanine dye (Cy-5), which minimized background fluorescence and scatter losses of the waveguide, was used in both assays. To our knowledge, this is the first report of femtomolar sensitivity in an immunosensing instrument.

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Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

January 1996

Volume

11

Issue

1-2

Start / End Page

149 / 160

Related Subject Headings

  • Silicon Compounds
  • Sensitivity and Specificity
  • Proteins
  • Optics and Photonics
  • Microchemistry
  • Ligands
  • Fluoroimmunoassay
  • Evaluation Studies as Topic
  • Biosensing Techniques
  • Bioinformatics
 

Citation

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Plowman, T. E., Reichert, W. M., Peters, C. R., Wang, H. K., Christensen, D. A., & Herron, J. N. (1996). Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor. Biosensors & Bioelectronics, 11(1–2), 149–160. https://doi.org/10.1016/0956-5663(96)83722-5
Plowman, T. E., W. M. Reichert, C. R. Peters, H. K. Wang, D. A. Christensen, and J. N. Herron. “Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor.Biosensors & Bioelectronics 11, no. 1–2 (January 1996): 149–60. https://doi.org/10.1016/0956-5663(96)83722-5.
Plowman TE, Reichert WM, Peters CR, Wang HK, Christensen DA, Herron JN. Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor. Biosensors & bioelectronics. 1996 Jan;11(1–2):149–60.
Plowman, T. E., et al. “Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor.Biosensors & Bioelectronics, vol. 11, no. 1–2, Jan. 1996, pp. 149–60. Epmc, doi:10.1016/0956-5663(96)83722-5.
Plowman TE, Reichert WM, Peters CR, Wang HK, Christensen DA, Herron JN. Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor. Biosensors & bioelectronics. 1996 Jan;11(1–2):149–160.
Journal cover image

Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

January 1996

Volume

11

Issue

1-2

Start / End Page

149 / 160

Related Subject Headings

  • Silicon Compounds
  • Sensitivity and Specificity
  • Proteins
  • Optics and Photonics
  • Microchemistry
  • Ligands
  • Fluoroimmunoassay
  • Evaluation Studies as Topic
  • Biosensing Techniques
  • Bioinformatics