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An autonomously self-assembling dendritic DNA nanostructure for target DNA detection.

Publication ,  Journal Article
Chandran, H; Rangnekar, A; Shetty, G; Schultes, EA; Reif, JH; LaBean, TH
Published in: Biotechnology journal
February 2013

There is a growing need for sensitive and reliable nucleic acid detection methods that are convenient and inexpensive. Responsive and programmable DNA nanostructures have shown great promise as chemical detection systems. Here, we describe a DNA detection system employing the triggered self-assembly of a novel DNA dendritic nanostructure. The detection protocol is executed autonomously without external intervention. Detection begins when a specific, single-stranded target DNA strand (T) triggers a hybridization chain reaction (HCR) between two, distinct DNA hairpins (α and β). Each hairpin opens and hybridizes up to two copies of the other. In the absence of T, α and β are stable and remain in their poised, closed-hairpin form. In the presence of T, α hairpins are opened by toe-hold mediated strand-displacement, each of which then opens and hybridizes two β hairpins. Likewise, each opened β hairpin can open and hybridize two α hairpins. Hence, each layer of the growing dendritic nanostructure can in principle accommodate an exponentially increasing number of cognate molecules, generating a high molecular weight nanostructure. This HCR system has minimal sequence constraints, allowing reconfiguration for the detection of arbitrary target sequences. Here, we demonstrate detection of unique sequence identifiers of HIV and Chlamydia pathogens.

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Published In

Biotechnology journal

DOI

EISSN

1860-7314

ISSN

1860-6768

Publication Date

February 2013

Volume

8

Issue

2

Start / End Page

221 / 227

Related Subject Headings

  • Sequence Analysis, DNA
  • Nucleic Acid Hybridization
  • Nucleic Acid Conformation
  • Metal Nanoparticles
  • HIV
  • Gold
  • Electrophoresis, Polyacrylamide Gel
  • DNA
  • Chlamydia
  • Biotechnology
 

Citation

APA
Chicago
ICMJE
MLA
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Chandran, H., Rangnekar, A., Shetty, G., Schultes, E. A., Reif, J. H., & LaBean, T. H. (2013). An autonomously self-assembling dendritic DNA nanostructure for target DNA detection. Biotechnology Journal, 8(2), 221–227. https://doi.org/10.1002/biot.201100499
Chandran, Harish, Abhijit Rangnekar, Geetha Shetty, Erik A. Schultes, John H. Reif, and Thomas H. LaBean. “An autonomously self-assembling dendritic DNA nanostructure for target DNA detection.Biotechnology Journal 8, no. 2 (February 2013): 221–27. https://doi.org/10.1002/biot.201100499.
Chandran H, Rangnekar A, Shetty G, Schultes EA, Reif JH, LaBean TH. An autonomously self-assembling dendritic DNA nanostructure for target DNA detection. Biotechnology journal. 2013 Feb;8(2):221–7.
Chandran, Harish, et al. “An autonomously self-assembling dendritic DNA nanostructure for target DNA detection.Biotechnology Journal, vol. 8, no. 2, Feb. 2013, pp. 221–27. Epmc, doi:10.1002/biot.201100499.
Chandran H, Rangnekar A, Shetty G, Schultes EA, Reif JH, LaBean TH. An autonomously self-assembling dendritic DNA nanostructure for target DNA detection. Biotechnology journal. 2013 Feb;8(2):221–227.
Journal cover image

Published In

Biotechnology journal

DOI

EISSN

1860-7314

ISSN

1860-6768

Publication Date

February 2013

Volume

8

Issue

2

Start / End Page

221 / 227

Related Subject Headings

  • Sequence Analysis, DNA
  • Nucleic Acid Hybridization
  • Nucleic Acid Conformation
  • Metal Nanoparticles
  • HIV
  • Gold
  • Electrophoresis, Polyacrylamide Gel
  • DNA
  • Chlamydia
  • Biotechnology