An autonomously self-assembling dendritic DNA nanostructure for target DNA detection.

Published

Journal Article

There is a growing need for sensitive and reliable nucleic acid detection methods that are convenient and inexpensive. Responsive and programmable DNA nanostructures have shown great promise as chemical detection systems. Here, we describe a DNA detection system employing the triggered self-assembly of a novel DNA dendritic nanostructure. The detection protocol is executed autonomously without external intervention. Detection begins when a specific, single-stranded target DNA strand (T) triggers a hybridization chain reaction (HCR) between two, distinct DNA hairpins (α and β). Each hairpin opens and hybridizes up to two copies of the other. In the absence of T, α and β are stable and remain in their poised, closed-hairpin form. In the presence of T, α hairpins are opened by toe-hold mediated strand-displacement, each of which then opens and hybridizes two β hairpins. Likewise, each opened β hairpin can open and hybridize two α hairpins. Hence, each layer of the growing dendritic nanostructure can in principle accommodate an exponentially increasing number of cognate molecules, generating a high molecular weight nanostructure. This HCR system has minimal sequence constraints, allowing reconfiguration for the detection of arbitrary target sequences. Here, we demonstrate detection of unique sequence identifiers of HIV and Chlamydia pathogens.

Full Text

Duke Authors

Cited Authors

  • Chandran, H; Rangnekar, A; Shetty, G; Schultes, EA; Reif, JH; LaBean, TH

Published Date

  • February 2013

Published In

Volume / Issue

  • 8 / 2

Start / End Page

  • 221 - 227

PubMed ID

  • 22965937

Pubmed Central ID

  • 22965937

Electronic International Standard Serial Number (EISSN)

  • 1860-7314

International Standard Serial Number (ISSN)

  • 1860-6768

Digital Object Identifier (DOI)

  • 10.1002/biot.201100499

Language

  • eng