Skip to main content

Activation by saccharides of a cation-selective pathway on canine lingual epithelium.

Publication ,  Journal Article
Simon, SA; Labarca, P; Robb, R
Published in: Am J Physiol
February 1989

Responses of isolated canine lingual epithelium in an Ussing chamber to D-glucose and fructose reveal events associated with taste transduction. With the use of isotopic flux studies, together with ion substitution and pharmacological and voltage clamp measurements, it was found that the stimulation of ion transport by D-glucose arises from an increase in the influx of cations through a cation-selective pathway. This influx of cations is completely inhibited by 0.1 mM amiloride. The stimulation of transport by fructose in 0.05 M KCl and by D-glucose in 0.05 M RbCl was also inhibited by amiloride, demonstrating that the saccharide-stimulated entry pathway was specific for neither hexose sugars nor for Na. Saccharide stimulation of canine lingual epithelia does not appear to be modulated by increases in intracellular levels of adenosine 3',5'-cyclic monophosphate, guanosine 3',5'-cyclic monophosphate, or Ca. The Na that enters taste cells on saccharide stimulation exits them via the ouabain inhibitable Na+-K+-adenosinetriphosphatase located in the serosal membranes.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1989

Volume

256

Issue

2 Pt 2

Start / End Page

R394 / R402

Location

United States

Related Subject Headings

  • Tongue
  • Taste
  • Sodium Chloride
  • Sodium
  • Signal Transduction
  • Ouabain
  • Mouth Mucosa
  • In Vitro Techniques
  • Glucose
  • Epithelium
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Simon, S. A., Labarca, P., & Robb, R. (1989). Activation by saccharides of a cation-selective pathway on canine lingual epithelium. Am J Physiol, 256(2 Pt 2), R394–R402. https://doi.org/10.1152/ajpregu.1989.256.2.R394
Simon, S. A., P. Labarca, and R. Robb. “Activation by saccharides of a cation-selective pathway on canine lingual epithelium.Am J Physiol 256, no. 2 Pt 2 (February 1989): R394–402. https://doi.org/10.1152/ajpregu.1989.256.2.R394.
Simon SA, Labarca P, Robb R. Activation by saccharides of a cation-selective pathway on canine lingual epithelium. Am J Physiol. 1989 Feb;256(2 Pt 2):R394–402.
Simon, S. A., et al. “Activation by saccharides of a cation-selective pathway on canine lingual epithelium.Am J Physiol, vol. 256, no. 2 Pt 2, Feb. 1989, pp. R394–402. Pubmed, doi:10.1152/ajpregu.1989.256.2.R394.
Simon SA, Labarca P, Robb R. Activation by saccharides of a cation-selective pathway on canine lingual epithelium. Am J Physiol. 1989 Feb;256(2 Pt 2):R394–R402.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1989

Volume

256

Issue

2 Pt 2

Start / End Page

R394 / R402

Location

United States

Related Subject Headings

  • Tongue
  • Taste
  • Sodium Chloride
  • Sodium
  • Signal Transduction
  • Ouabain
  • Mouth Mucosa
  • In Vitro Techniques
  • Glucose
  • Epithelium