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Identification of a novel inositol bisphosphate isomer formed in chemoattractant stimulated human polymorphonuclear leukocytes.

Publication ,  Journal Article
Dillon, SB; Murray, JJ; Snyderman, R
Published in: Biochem Biophys Res Commun
April 14, 1987

Analysis of inositol phosphate formation in chemoattractant-stimulated human polymorphonuclear leukocytes demonstrated the production of inositol 1,4,5-trisphosphate, inositol 1,3,4-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, inositol 1,4-bisphosphate and another inositol bisphosphate isomer not detected in unstimulated cells. Studies in cell sonicates provided evidence that the previously unidentified inositol bisphosphate isomer is produced via the degradation of inositol 1,3,4-trisphosphate. This unidentified inositol bisphosphate peak was purified by high pressure liquid chromatography, and base hydrolyzed to form a mixture of inositol monophosphate isomers. Based on these studies, the unidentified peak was identified as inositol 3,4-bisphosphate. Identification of this isomer defines a new metabolic product derived from the initial inositol 1,4,5-trisphosphate formation, and also suggests another substrate for the inositol 1-phosphatase.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

April 14, 1987

Volume

144

Issue

1

Start / End Page

264 / 270

Location

United States

Related Subject Headings

  • Type C Phospholipases
  • Sugar Phosphates
  • Protein Kinase C
  • Phosphoric Monoester Hydrolases
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • Isomerism
  • Inositol Phosphates
  • Inositol 1,4,5-Trisphosphate
  • In Vitro Techniques
 

Citation

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Dillon, S. B., Murray, J. J., & Snyderman, R. (1987). Identification of a novel inositol bisphosphate isomer formed in chemoattractant stimulated human polymorphonuclear leukocytes. Biochem Biophys Res Commun, 144(1), 264–270. https://doi.org/10.1016/s0006-291x(87)80505-3
Dillon, S. B., J. J. Murray, and R. Snyderman. “Identification of a novel inositol bisphosphate isomer formed in chemoattractant stimulated human polymorphonuclear leukocytes.Biochem Biophys Res Commun 144, no. 1 (April 14, 1987): 264–70. https://doi.org/10.1016/s0006-291x(87)80505-3.
Dillon SB, Murray JJ, Snyderman R. Identification of a novel inositol bisphosphate isomer formed in chemoattractant stimulated human polymorphonuclear leukocytes. Biochem Biophys Res Commun. 1987 Apr 14;144(1):264–70.
Dillon, S. B., et al. “Identification of a novel inositol bisphosphate isomer formed in chemoattractant stimulated human polymorphonuclear leukocytes.Biochem Biophys Res Commun, vol. 144, no. 1, Apr. 1987, pp. 264–70. Pubmed, doi:10.1016/s0006-291x(87)80505-3.
Dillon SB, Murray JJ, Snyderman R. Identification of a novel inositol bisphosphate isomer formed in chemoattractant stimulated human polymorphonuclear leukocytes. Biochem Biophys Res Commun. 1987 Apr 14;144(1):264–270.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

April 14, 1987

Volume

144

Issue

1

Start / End Page

264 / 270

Location

United States

Related Subject Headings

  • Type C Phospholipases
  • Sugar Phosphates
  • Protein Kinase C
  • Phosphoric Monoester Hydrolases
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • Isomerism
  • Inositol Phosphates
  • Inositol 1,4,5-Trisphosphate
  • In Vitro Techniques