Molecular cloning of a new human G protein. Evidence for two Gi alpha-like protein families.
Journal Article (Journal Article)
The amino acid sequence of a novel G protein alpha subunit (Gx alpha) has been deduced from the nucleotide sequence of a human cDNA clone isolated from a differentiated HL-60 cDNA library. The cDNA encodes a polypeptide of 354 amino acids (Mr 40,519) which is closely related to Gi alpha proteins. The amino acid sequence homology between Gx alpha and human myeloid Gi alpha is 86% with 15 nonconservative substitutions. Gx alpha also shares 86% homology with both rat brain and mouse macrophage Gi alpha but is more homologous (94%) to bovine brain Gi alpha with only 5 nonconservative amino acid differences. G proteins previously termed Gi alpha may fall into at least two distinct groups, with one including human myeloid Gi alpha, rat brain Gi alpha and mouse macrophage Gi alpha; and other Gx alpha and bovine brain Gi alpha. One group probably contains true Gi and the other a new class of G protein whose function remains to be determined.
Full Text
Duke Authors
Cited Authors
- Didsbury, JR; Snyderman, R
Published Date
- July 13, 1987
Published In
Volume / Issue
- 219 / 1
Start / End Page
- 259 - 263
PubMed ID
- 3109953
International Standard Serial Number (ISSN)
- 0014-5793
Digital Object Identifier (DOI)
- 10.1016/0014-5793(87)81228-0
Language
- eng
Conference Location
- England