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Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein.

Publication ,  Journal Article
Smith, CD; Cox, CC; Snyderman, R
Published in: Science
April 4, 1986

Cleavage of phosphatidylinositol 4,5-bisphosphate by phospholipase C results in the production of two important second messengers: inositol-1,4,5-trisphosphate and 1,2-diacylglycerol. Although several receptors promote this cleavage, the molecular details of phospholipase C activation have remained unresolved. In this study, occupancy of a Ca2+-mobilizing receptor, the oligopeptide chemoattractant receptor on human polymorphonuclear leukocyte plasma membranes, was found to lead to the activation of a guanine nucleotide regulatory (N) protein by guanosine 5'-triphosphate. The activated N protein then stimulated a polyphosphoinositide-specific phospholipase C by reducing the Ca2+ requirement for expression of this activity from superphysiological to normal intracellular concentrations. Therefore, the N protein-mediated activation of phospholipase C may be a key step in the pathway of cellular activation by chemoattractants and certain other hormones.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

April 4, 1986

Volume

232

Issue

4746

Start / End Page

97 / 100

Location

United States

Related Subject Headings

  • Type C Phospholipases
  • Ribonucleotides
  • Phosphorus Radioisotopes
  • Phosphatidylinositols
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • Kinetics
  • Humans
  • General Science & Technology
  • GTP-Binding Proteins
 

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Smith, C. D., Cox, C. C., & Snyderman, R. (1986). Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein. Science, 232(4746), 97–100. https://doi.org/10.1126/science.3006254
Smith, C. D., C. C. Cox, and R. Snyderman. “Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein.Science 232, no. 4746 (April 4, 1986): 97–100. https://doi.org/10.1126/science.3006254.
Smith CD, Cox CC, Snyderman R. Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein. Science. 1986 Apr 4;232(4746):97–100.
Smith, C. D., et al. “Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein.Science, vol. 232, no. 4746, Apr. 1986, pp. 97–100. Pubmed, doi:10.1126/science.3006254.
Smith CD, Cox CC, Snyderman R. Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein. Science. 1986 Apr 4;232(4746):97–100.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

April 4, 1986

Volume

232

Issue

4746

Start / End Page

97 / 100

Location

United States

Related Subject Headings

  • Type C Phospholipases
  • Ribonucleotides
  • Phosphorus Radioisotopes
  • Phosphatidylinositols
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • Kinetics
  • Humans
  • General Science & Technology
  • GTP-Binding Proteins