Transmethylation reactions regulate affinity and functional activity of chemotactic factor receptors on macrophages.

Published

Journal Article

Methylation mediated by S-adenosyl-L-methionine is required for the chemotaxis of mononuclear leukocytes. We investigated whether transmethylation reactions are required for normal functioning of chemotactic factor receptors. Three chemoatractant-mediated functions in macrophages, chemotaxis, the stimulated release of arachidonic acid from membrane phospholipids and superoxide production, are markedly depressed by agents that inhibit cellular methylation reactions. Treatment of macrophages with methylation inhibitors decreased the affinity of the N-formylated chemoattractant receptor present on these cells by a factor of 4.5, but did not significantly alter the total receptor on macrophages can exist in more than one affinity state and that an ongoing methylation reaction is required for the maintenance of the receptor in its higher affinity form. Inhibition of methylation lowers the affinity of the receptor and renders it nonfunctional or "uncoupled" in its ability to produce chemotaxis, superoxide and the release of a arachidonic acid from leukocyte membranes.

Full Text

Duke Authors

Cited Authors

  • Pike, MC; Snyderman, R

Published Date

  • January 1, 1982

Published In

Volume / Issue

  • 28 / 1

Start / End Page

  • 107 - 114

PubMed ID

  • 6279308

Pubmed Central ID

  • 6279308

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(82)90380-4

Language

  • eng

Conference Location

  • United States