Neutralization of sensitized virus by purified components of complement.

Published

Journal Article

Herpes simplex virus which had been sensitized with immunoglobulin M antibody was neutralized by serum deficient in the fifth and sixth components of complement (C) but not by serum deficient in the fourth component C (C4). The sequential addition of the functionally purified components of C showed that the activated first component of C (C1[unk]) failed to neutralize sensitized virus. However, in the presence of an optimal concentration of C1[unk], the addition of C4 resulted in neutralization. The amount of virus neutralized was dependent upon the concentration of immunoglobulin M used to sensitize the virus and the concentration of C1[unk] and C4. The addition of the second component of C (C2) to reaction mixtures containing an optimal concentration of C1[unk] and a limiting concentration of C4 resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C2. The addition of the third component of C (C3) to reaction mixtures containing an optimal concentration of C1[unk] and limiting concentrations of C4 and C2 also resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C3.

Full Text

Duke Authors

Cited Authors

  • Daniels, CA; Borsos, T; Rapp, HJ; Snyderman, R; Notkins, AL

Published Date

  • March 1, 1970

Published In

Volume / Issue

  • 65 / 3

Start / End Page

  • 528 - 535

PubMed ID

  • 4315612

Pubmed Central ID

  • 4315612

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.65.3.528

Language

  • eng

Conference Location

  • United States