Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells.

Journal Article (Journal Article)

Exocytosis is initiated by the receptor-mediated influx of calcium that results in fusion of the secretory vesicle with the plasma membrane. We examined the possibility that calcium-dependent exocytosis in mast cells and adrenal chromaffin cells requires metalloendoprotease activity. Metalloendoprotease inhibitors and dipeptide substrates block exocytosis in these cells with the same specificity and dose dependency as that with which they interact with metalloendoproteases. Metalloendoprotease activity is identified in these cells with fluorogenic synthetic substrates, which also blocked exocytosis. Metalloendoprotease activity is highest in the plasma membrane of chromaffin cells. The metalloendoprotease appears to be required in exocytosis at a step dependent on or after calcium entry, since exocytosis initiated by direct calcium introduction in both mast cells and chromaffin cells is blocked by metalloendoprotease inhibitors.

Full Text

Duke Authors

Cited Authors

  • Mundy, DI; Strittmatter, WJ

Published Date

  • March 1, 1985

Published In

Volume / Issue

  • 40 / 3

Start / End Page

  • 645 - 656

PubMed ID

  • 2578889

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(85)90213-2


  • eng

Conference Location

  • United States