ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream activation sequences.


Journal Article

The ACE1 protein of Saccharomyces cerevisiae was expressed as a trpE-ACE1 fusion protein in Escherichia coli and shown to bind CUP1 upstream activation sequences at multiple regions in a copper-inducible manner. These binding sites contain within them the sequence 5'-TC(T)4-6GCTG-3', which we propose constitutes an important part of the ACE1 consensus recognition sequence.

Full Text

Duke Authors

Cited Authors

  • Evans, CF; Engelke, DR; Thiele, DJ

Published Date

  • January 1990

Published In

Volume / Issue

  • 10 / 1

Start / End Page

  • 426 - 429

PubMed ID

  • 2403647

Pubmed Central ID

  • 2403647

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.10.1.426


  • eng

Conference Location

  • United States