Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences.


Journal Article

Copper-inducible transcription of the yeast metallothionein gene (CUP1) occurs by means of cis-acting upstream activation sequences (UAS) and trans-acting cellular factors. We have used a high-resolution chromosomal footprinting technique to detect the interaction of cellular factors with UASCUP1. Our results demonstrate that one or more cellular factors bind to UASCUP1 in a copper-inducible fashion. This copper-inducible binding is enhanced in a yeast strain that harbors several copies of the positive regulatory gene ACE1 and is not detectable in yeast cells that contain a nonfunctional (ace1-delta 1) locus. The correlation between yeast metallothionein gene activation and copper-inducible occupation of UASCUP1 sequences suggests that the binding of metallothionein transcriptional regulatory factors to cis-acting control sequences is copper-inducible.

Full Text

Duke Authors

Cited Authors

  • Huibregtse, JM; Engelke, DR; Thiele, DJ

Published Date

  • January 1989

Published In

Volume / Issue

  • 86 / 1

Start / End Page

  • 65 - 69

PubMed ID

  • 2643107

Pubmed Central ID

  • 2643107

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.86.1.65


  • eng

Conference Location

  • United States