Saccharomyces cerevisiae killer virus transcripts contain template-coded polyadenylate tracts.


Journal Article

The M double-stranded RNA component of type 1 killer strains of the yeast Saccharomyces cerevisiae contains an internal 200-base pair adenine- and uracil-rich region. The plus strands of this viral genomic RNA contain an internal adenine-rich region which allows these strands to bind to polyuridylate-Sepharose as tightly as do polyadenylated RNAs with 3'-terminal polyadenylated tracts of 70 to 100 residues. Internal template coding of an adenine-rich tract in positive polarity in vivo and in vitro transcripts of M double-stranded RNA may serve as an alternate method of transcript polyadenylation. The 3'-terminal residue of the in vitro m transcript is a non-template-encoded purine residue. The 5' terminus of this transcript is involved in a stem-and-loop structure which includes an AUG initiation codon, along with potential 18S and 5.8S rRNA binding sites. Except for the 3'-terminal residue, transcription in in vitro shows complete fidelity.

Full Text

Duke Authors

Cited Authors

  • Hannig, EM; Thiele, DJ; Leibowitz, MJ

Published Date

  • January 1, 1984

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 101 - 109

PubMed ID

  • 6199660

Pubmed Central ID

  • 6199660

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.4.1.101


  • eng

Conference Location

  • United States