Separation and sequence of the 3' termini of M double-stranded RNA from killer yeast.

Published

Journal Article

Four subspecies of M double-stranded RNA from a killer strain of Saccharomyces cerevisiae were isolated. Each subspecies were susceptible to heat cleavage, presumably at an internal 190 base pair A,U-rich region, generating two discrete fragments corresponding to each side of the A, U-rich region. Enzymatic and chemical RNA sequence analysis defined the 3'-terminal 175 bases for the larger fragment (M-1) and 231 bases for the smaller fragment (M-2). All four subspecies of M have identical size and 3'-terminal sequences. Potential translation initiation codons are present on the corresponding 5' termini of both fragments, and a possible 18S ribosomal RNA binding site is also present on the 5' terminus of M-1. Stem and loop structures for the 5' and 3' termini of M-1 may function as recognition sites for replication, transcription, and translation.

Full Text

Duke Authors

Cited Authors

  • Thiele, DJ; Wang, RW; Leibowitz, MJ

Published Date

  • March 11, 1982

Published In

Volume / Issue

  • 10 / 5

Start / End Page

  • 1661 - 1678

PubMed ID

  • 7041093

Pubmed Central ID

  • 7041093

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/10.5.1661

Language

  • eng

Conference Location

  • England