Localization of a peptide identified by antibodies to gastrin/CCK in the gut of Cancer magister


Journal Article

A gastric peptide from the Dungeness crab (Cancer magister), extracted and characterized previously (Larson and Vigna 1983b), was localized in the foregut (stomach) of this species by immunocytochemistry using antisera specific for the bioactive carboxy-terminal amino acid sequence common to gastrins and cholecystokinins (CCKs). Immunoreactivity was found in all gastric epithelial cells and in the procuticle. Electron microscopy revealed an absence of peptidergic secretory granules in the gastric epithelial cells. The pattern of immunostaining suggests that the gastric epithelial cells secrete this peptide apically where it is incorporated into the cuticle lining the lumen. Specific immunostaining could not be demonstrated in various neural ganglia or in the hypodermis. The distribution of this peptide is different from that of gastrin/CCK in vertebrates and other invertebrates. This suggests that the crab gastric peptide is sufficiently similar to gastrin/CCK to react with C-terminal specific antisera, but may be anatomically, functionally, and possibly phylogenetically otherwise unrelated. © 1984 Springer-Verlag.

Full Text

Duke Authors

Cited Authors

  • Scalise, FW; Larson, BA; Vigna, SR

Published Date

  • September 1, 1984

Published In

Volume / Issue

  • 238 / 1

Start / End Page

  • 113 - 119

Electronic International Standard Serial Number (EISSN)

  • 1432-0878

International Standard Serial Number (ISSN)

  • 0302-766X

Digital Object Identifier (DOI)

  • 10.1007/BF00215151

Citation Source

  • Scopus