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A pathway of neuronal apoptosis induced by hypoxia/reoxygenation: roles of nuclear factor-kappaB and Bcl-2.

Publication ,  Journal Article
Tamatani, M; Mitsuda, N; Matsuzaki, H; Okado, H; Miyake, S; Vitek, MP; Yamaguchi, A; Tohyama, M
Published in: J Neurochem
August 2000

As a model of the reperfusion injury found in stroke, we have exposed neurons to hypoxia followed by reoxygenation. Neurons treated with hypoxia/reoxygenation (H/R) respond by activating nuclear factor-kappaB (NFkappaB), releasing cytochrome c from their mitochondria, and ultimately dying. Further supporting an apoptotic mechanism, expression of the antiapoptotic Bcl-2 and Bcl-x proteins was increased following H/R. In this model, adenoviral-mediated transduction of lkappaB expression inhibited NFkappaB activation and significantly accelerated cytochrome c release and caspase-dependent neuronal death. At the same time, expression of mutated lkappaB prevented the increased expression of endogenous Bcl-2 and Bcl-x. In the presence of mutated lkappaB, singular overexpression of only Bcl-2 by adenoviral-mediated transduction significantly inhibited cytochrome c release, caspase-3-like activation, and cell death in response to H/R. These findings suggest a pathway where NFkappaB activation induces overexpression of Bcl-2 and Bcl-x, which function to prevent apoptotic cell death following H/R treatments.

Duke Scholars

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

August 2000

Volume

75

Issue

2

Start / End Page

683 / 693

Location

England

Related Subject Headings

  • bcl-X Protein
  • bcl-2-Associated X Protein
  • Transcription, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats, Sprague-Dawley
  • Rats
  • Proto-Oncogene Proteins c-bcl-2
  • Proto-Oncogene Proteins
  • Neurons
  • Neurology & Neurosurgery
 

Citation

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MLA
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Tamatani, M., Mitsuda, N., Matsuzaki, H., Okado, H., Miyake, S., Vitek, M. P., … Tohyama, M. (2000). A pathway of neuronal apoptosis induced by hypoxia/reoxygenation: roles of nuclear factor-kappaB and Bcl-2. J Neurochem, 75(2), 683–693. https://doi.org/10.1046/j.1471-4159.2000.0750683.x
Tamatani, M., N. Mitsuda, H. Matsuzaki, H. Okado, S. Miyake, M. P. Vitek, A. Yamaguchi, and M. Tohyama. “A pathway of neuronal apoptosis induced by hypoxia/reoxygenation: roles of nuclear factor-kappaB and Bcl-2.J Neurochem 75, no. 2 (August 2000): 683–93. https://doi.org/10.1046/j.1471-4159.2000.0750683.x.
Tamatani M, Mitsuda N, Matsuzaki H, Okado H, Miyake S, Vitek MP, et al. A pathway of neuronal apoptosis induced by hypoxia/reoxygenation: roles of nuclear factor-kappaB and Bcl-2. J Neurochem. 2000 Aug;75(2):683–93.
Tamatani, M., et al. “A pathway of neuronal apoptosis induced by hypoxia/reoxygenation: roles of nuclear factor-kappaB and Bcl-2.J Neurochem, vol. 75, no. 2, Aug. 2000, pp. 683–93. Pubmed, doi:10.1046/j.1471-4159.2000.0750683.x.
Tamatani M, Mitsuda N, Matsuzaki H, Okado H, Miyake S, Vitek MP, Yamaguchi A, Tohyama M. A pathway of neuronal apoptosis induced by hypoxia/reoxygenation: roles of nuclear factor-kappaB and Bcl-2. J Neurochem. 2000 Aug;75(2):683–693.
Journal cover image

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

August 2000

Volume

75

Issue

2

Start / End Page

683 / 693

Location

England

Related Subject Headings

  • bcl-X Protein
  • bcl-2-Associated X Protein
  • Transcription, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats, Sprague-Dawley
  • Rats
  • Proto-Oncogene Proteins c-bcl-2
  • Proto-Oncogene Proteins
  • Neurons
  • Neurology & Neurosurgery