Is amyloid beta-protein glycated in Alzheimer's disease?
Journal Article (Journal Article)
Recent data suggest that protein glycation is involved in the process of amyloid formation in Alzheimer's disease (AD). To further investigate this issue, we analyzed the presence of advanced glycation end products (AGE) in soluble and insoluble forms of amyloid beta-protein (A beta) as well as in apolipoprotein E (apoE), a protein bound to amyloid deposits. Both proteins were extracted from cerebral cortex obtained from patients with AD and probed by immunoblotting with two antibodies specific for different AGE, already known to immunocytochemically label amyloid plaques. All the AGE antibodies failed to recognize either A beta or apoE, whereas they reacted with synthetic A beta glycated in vitro. These findings indicate that other proteins associated with amyloid deposits are candidates to be modified with AGE in Alzheimer's cerebral tissue.
Full Text
Duke Authors
Cited Authors
- Tabaton, M; Perry, G; Smith, M; Vitek, M; Angelini, G; Dapino, D; Garibaldi, S; Zaccheo, D; Odetti, P
Published Date
- March 3, 1997
Published In
Volume / Issue
- 8 / 4
Start / End Page
- 907 - 909
PubMed ID
- 9141062
International Standard Serial Number (ISSN)
- 0959-4965
Digital Object Identifier (DOI)
- 10.1097/00001756-199703030-00018
Language
- eng
Conference Location
- England