Surface-enhanced Raman scattering detection of the breast cancer susceptibility gene BRCA1 using a silver-coated microarray platform


Journal Article

Surface-enhanced Raman scattering (SERS) spectroscopy was used to monitor DNA hybridization of a fragment of the BRCA1 breast cancer susceptibility gene on modified silver surfaces. Rhodamine B was covalently attached to a 5′-amino-labeled oligonucleotide sequence (23 mer) through a succinimidyl ester intermediate in methanol. The silver surfaces were prepared by depositing a discontinuous layer (9.0nm) of silver onto glass slides, which had been etched with HF to form a microwell platform, and subsequently modified with a monolayer of mercaptoundecanoic acid. The complementary probe was covalently attached to the silver surfaces using a succinimidyl ester intermediate in acetonitrile. The silver island substrate allows a very large enhancement of the Raman signal of the DNA-Rhodamine B, and clear distinction between hybridized samples and controls on a microwell array sampling platform. © 2002 Elsevier Science B.V. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • Allain, LR; Vo-Dinh, T

Published Date

  • September 26, 2002

Published In

Volume / Issue

  • 469 / 1

Start / End Page

  • 149 - 154

International Standard Serial Number (ISSN)

  • 0003-2670

Digital Object Identifier (DOI)

  • 10.1016/S0003-2670(01)01537-9

Citation Source

  • Scopus