Simultaneous detection of the tumor suppressor FHIT gene and protein using the multi-functional biochip.

Published

Journal Article

The tumor suppressor gene, fragile histidine triad (FHIT), encompasses the most common human chromosomal fragile site, at 3pl4.2. Detection of FHIT gene is important in cancer diagnostics since its alterations have been associated with several human cancers. A unique multi-functional biochip for simultaneous detection of FHIT DNA and FHIT protein on the same platform was applied. The design of the biochip is based on miniaturization of photodiodes, where functioning of multiple optical sensing elements, amplifiers, discriminators, and logic circuitry are integrated on a single IC board. Performance of biochip is based on biomolecular recognition processes using both DNA and protein bioreceptors, Cy5-labeled probes and laser excitation. Application of biochip for concurrent detection of various immobilized target DNA and protein molecules and multiplex of DNA and protein on the same microarray was accomplished. Linearity of biochip for quantitative measurements was demonstrated. Results demonstrated utility of this multi-functional biochip as a useful detection technology with applications in biological and clinical laboratories.

Full Text

Duke Authors

Cited Authors

  • Askari, MDF; Miller, GH; Vo-Dinh, T

Published Date

  • January 2002

Published In

Volume / Issue

  • 26 / 5

Start / End Page

  • 331 - 342

PubMed ID

  • 12518863

Pubmed Central ID

  • 12518863

Electronic International Standard Serial Number (EISSN)

  • 1525-1500

International Standard Serial Number (ISSN)

  • 0361-090X

Digital Object Identifier (DOI)

  • 10.1016/s0361-090x(02)00091-0

Language

  • eng