Skip to main content

Microarray sampling-platform fabrication using bubble-jet technology for a biochip system.

Publication ,  Journal Article
Allain, LR; Askari, M; Stokes, DL; Vo-Dinh, T
Published in: Fresenius' journal of analytical chemistry
September 2001

The fabrication of microarrays containing PCR-amplified genomic DNA extracts from mice tumors on a Zetaprobe membrane using a modified thermal ink-jet printer is described. A simple and cost-effective procedure for the fabrication of microarrays containing biological samples using a modified bubble-jet printing system is presented. Because of their mass-produced design, ink-jet printers are a much cheaper alternative to conventional spotting techniques. The usefulness of the biochip microarray platform is illustrated by the detection of human fragile histidine triad (FHIT), a tumor suppressor gene. Subcutaneous carcinomas were induced with MKN/FHIT and MKN/E4 cell lines in immunodeficient mice. Several weeks into their development, the tumors from both groups of mice were removed and subjected to DNA extraction by lysis of tissue samples. The extracted DNA samples were amplified by PCR (30 cycles) using the primers corresponding to nucleotides 2 to 18 of the FHIT sequence. The resulting solution was transferred to the individual reservoirs of a three-color cartridge from a conventional thermal ink-jet printer (HP 694C), and arrays were printed on to a Zetaprobe membrane. After spotting, these membranes were used in a hybridization assay, using fluorescent probes, and detected with a biochip.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Fresenius' journal of analytical chemistry

DOI

EISSN

1432-1130

ISSN

0937-0633

Publication Date

September 2001

Volume

371

Issue

2

Start / End Page

146 / 150

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Oligonucleotide Array Sequence Analysis
  • Nucleic Acid Hybridization
  • Neoplasms, Experimental
  • Neoplasm Proteins
  • Microchemistry
  • Mice
  • Humans
  • DNA, Neoplasm
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Allain, L. R., Askari, M., Stokes, D. L., & Vo-Dinh, T. (2001). Microarray sampling-platform fabrication using bubble-jet technology for a biochip system. Fresenius’ Journal of Analytical Chemistry, 371(2), 146–150. https://doi.org/10.1007/s002160100962
Allain, L. R., M. Askari, D. L. Stokes, and T. Vo-Dinh. “Microarray sampling-platform fabrication using bubble-jet technology for a biochip system.Fresenius’ Journal of Analytical Chemistry 371, no. 2 (September 2001): 146–50. https://doi.org/10.1007/s002160100962.
Allain LR, Askari M, Stokes DL, Vo-Dinh T. Microarray sampling-platform fabrication using bubble-jet technology for a biochip system. Fresenius’ journal of analytical chemistry. 2001 Sep;371(2):146–50.
Allain, L. R., et al. “Microarray sampling-platform fabrication using bubble-jet technology for a biochip system.Fresenius’ Journal of Analytical Chemistry, vol. 371, no. 2, Sept. 2001, pp. 146–50. Epmc, doi:10.1007/s002160100962.
Allain LR, Askari M, Stokes DL, Vo-Dinh T. Microarray sampling-platform fabrication using bubble-jet technology for a biochip system. Fresenius’ journal of analytical chemistry. 2001 Sep;371(2):146–150.

Published In

Fresenius' journal of analytical chemistry

DOI

EISSN

1432-1130

ISSN

0937-0633

Publication Date

September 2001

Volume

371

Issue

2

Start / End Page

146 / 150

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Oligonucleotide Array Sequence Analysis
  • Nucleic Acid Hybridization
  • Neoplasms, Experimental
  • Neoplasm Proteins
  • Microchemistry
  • Mice
  • Humans
  • DNA, Neoplasm