Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein

Journal Article (Journal Article)

Human fragile histidine triad (FHIT) protein has dinucleoside 5′,5‴-P1, Pn-polyphosphates hydrolysis activity, with AMP being one of the reaction products. Application of synchronous luminescence (SL) spectroscopy, in which both excitation and emission wavelengths are scanned simultaneously while a constant wavelength interval is maintained between them, was investigated for detection of the enzymatic activity of the FHIT protein. Ability of SL to identify reaction components, AMP production and its increase as a result of increase in substrate concentration and inhibition of the hydrolysis activity by ZnCl2 are demonstrated.

Full Text

Duke Authors

Cited Authors

  • Askari, M; Miller, G; Vo-Dinh, T

Published Date

  • January 1, 2001

Published In

Volume / Issue

  • 23 / 20

Start / End Page

  • 1697 - 1702

International Standard Serial Number (ISSN)

  • 0141-5492

Digital Object Identifier (DOI)

  • 10.1023/A:1012404430463

Citation Source

  • Scopus