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Development of a new capillary electrophoresis-based fibre optic sensor.

Publication ,  Journal Article
Stokes, DL; Sepaniak, MJ; Vo-Dinh, T
Published in: Biomedical chromatography : BMC
July 1997

A new fluorescence-based fibre optic sensor is described which combines the sensitivity offered by laser-induced fluorescence with the selectivity offered by capillary electrophoresis (CE). A single optical fibre directly probes the terminus of a 5-8 cm separation capillary. The linear geometry associated with this sensor necessitates a 'single reservoir' design, thus presenting major challenges to overcome in comparison to the conventional two-reservoir configuration common to a typical laboratory setup. Some of the challenges confronted by the design features presented in this work include the reduction of gravity-driven hydrostatic flow, the ejection of electrolytic gases evolved at the detection-side electrode and the establishment a suitable compromise between detectability and separation performance. The success of such design features demonstrates the feasibility of a CE-based sensor which offers several amenities particularly useful for in situ sensing. Such attributes include selectivity, diminutive size, flexibility, reusability, high sensitivity, speed, and remote control. Detailed descriptions of sensor fabrication are included, including two variations on a general design concept. In addition, the single-fibre optical detection system is described. Separation characteristics of the new CE-based sensor are presented, highlighted by an observed separation efficiency of up to 8000 theoretical plates (for a 5 cm capillary). The separation of a three-component mixture of the laser dyes, Rhodamine 6G, fluorescein isothyocyanate and sodium fluorescein, is demonstrated.

Duke Scholars

Published In

Biomedical chromatography : BMC

DOI

EISSN

1099-0801

ISSN

0269-3879

Publication Date

July 1997

Volume

11

Issue

4

Start / End Page

187 / 192

Related Subject Headings

  • Rhodamines
  • Lasers
  • Fluorescent Dyes
  • Fluoresceins
  • Fluorescein-5-isothiocyanate
  • Fluorescein
  • Fiber Optic Technology
  • Electrophoresis, Capillary
  • Biosensing Techniques
  • Analytical Chemistry
 

Citation

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ICMJE
MLA
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Stokes, D. L., Sepaniak, M. J., & Vo-Dinh, T. (1997). Development of a new capillary electrophoresis-based fibre optic sensor. Biomedical Chromatography : BMC, 11(4), 187–192. https://doi.org/10.1002/(sici)1099-0801(199707)11:4<187::aid-bmc671>3.0.co;2-0
Stokes, D. L., M. J. Sepaniak, and T. Vo-Dinh. “Development of a new capillary electrophoresis-based fibre optic sensor.Biomedical Chromatography : BMC 11, no. 4 (July 1997): 187–92. https://doi.org/10.1002/(sici)1099-0801(199707)11:4<187::aid-bmc671>3.0.co;2-0.
Stokes DL, Sepaniak MJ, Vo-Dinh T. Development of a new capillary electrophoresis-based fibre optic sensor. Biomedical chromatography : BMC. 1997 Jul;11(4):187–92.
Stokes, D. L., et al. “Development of a new capillary electrophoresis-based fibre optic sensor.Biomedical Chromatography : BMC, vol. 11, no. 4, July 1997, pp. 187–92. Epmc, doi:10.1002/(sici)1099-0801(199707)11:4<187::aid-bmc671>3.0.co;2-0.
Stokes DL, Sepaniak MJ, Vo-Dinh T. Development of a new capillary electrophoresis-based fibre optic sensor. Biomedical chromatography : BMC. 1997 Jul;11(4):187–192.
Journal cover image

Published In

Biomedical chromatography : BMC

DOI

EISSN

1099-0801

ISSN

0269-3879

Publication Date

July 1997

Volume

11

Issue

4

Start / End Page

187 / 192

Related Subject Headings

  • Rhodamines
  • Lasers
  • Fluorescent Dyes
  • Fluoresceins
  • Fluorescein-5-isothiocyanate
  • Fluorescein
  • Fiber Optic Technology
  • Electrophoresis, Capillary
  • Biosensing Techniques
  • Analytical Chemistry