Laser-induced fluorescence detection of dysplasia in Barrett's esophagus

Journal Article

A study was conducted to determine whether laser-induced fluorescence could detect high grade dysplasia in Barrett's esophagus. Four-hundred-ten nm laser light was used to induce autofluorescence of Barrett's mucosa in 36 patients during routine endoscopy. The spectra were analyzed using the Differential Normalized Fluorescence (DNF) Index technique to differentiate high grade dysplasia from either low grade or non-dysplastic mucosa. Each spectrum was classified as either premalignant or benign using two different DNF indices. Analyzing the fluorescence spectra from all patients using one DNF Index, 96% of non-dysplastic Barrett's samples classified as benign tissue. All low grade dysplasia samples classified as benign. Ninety percent of high grade dysplasia samples classified as premalignant. Twenty-eight percent of mixed low grade/focal high grade dysplasia samples classified as premalignant. In summary, high grade dysplasia in Barrett's esophagus patients can be detected by endoscopic laser-induced fluorescence spectroscopy using differential normalized fluorescence technique. ©2004 Copyright SPIE - The International Society for Optical Engineering.

Full Text

Duke Authors

Cited Authors

  • Panjehpour, M; Overholt, BF; Vo-Dinh, T; Edwards, DH; Buckley, PF; DeCosta, JF; Haggitt, RC

Published Date

  • December 1, 1996

Published In

Volume / Issue

  • 2679 /

Start / End Page

  • 16 - 25

International Standard Serial Number (ISSN)

  • 0277-786X

Digital Object Identifier (DOI)

  • 10.1117/12.237584

Citation Source

  • Scopus