Paper electrophoresis with surface-enhanced fluorimetric detection
Amorphous fumed silica was used as a fluorescence sensitizer coated on a filter-paper substrate for use in paper electrophoresis. The usefulness of the separation method was illustrated for a mixture of two polynuclear aromatic compounds of biological interest: benzo[a]pyrene (BaP) and benzo[a]pyrene-r-7,t-8,9,10-tetrahydrotetrol, a hydrolysis product of BaP-DNA adduct. These two compounds were well separated and an enhancement of the fluorescence intensity was also observed when the sensitizer was applied to filter-paper prior to electrophoretic separation. © 1990.
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