Fiber-Optic time-resolved fluorimetry for immunoassays.

Published

Journal Article

Rare-earth metal chelates used as fluorescent labels for immunoassays possess extremely long fluorescence lifetimes and permit the elective use of time-resolved detection. This is very important in fiber-optic fluorimetry, a technique that ordinarily exhibits large signal backgrounds from back-scattered radiation. With time-resolved detection to reject the back-scattered radiation, the limit of detection for Eu 2-naphthoyltrifluoroacetonate is 10(-12)M, nearly three orders of magnitude lower than for the fiber-optic measurement of the most common fluorescent label, fluorescein isothiocyanate. Commercially available reagents labeled with an europium chelate are used to demonstrate the potential utility of time-resolved fluorimetry in fiber-optic immunoassays. Rabbit immunoglobulin G (IgG) is covalently bonded to the distal end of quartz optical fibers prior to exposure to anti-rabbit IgG labelled with europium chelate. The limit of detection for the assay is approximately 0.1 mug/ml.

Full Text

Duke Authors

Cited Authors

  • Petrea, RD; Sepaniak, MJ; Vo-Dinh, T

Published Date

  • February 1988

Published In

Volume / Issue

  • 35 / 2

Start / End Page

  • 139 - 144

PubMed ID

  • 18964482

Pubmed Central ID

  • 18964482

Electronic International Standard Serial Number (EISSN)

  • 1873-3573

International Standard Serial Number (ISSN)

  • 0039-9140

Language

  • eng