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Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase.

Publication ,  Journal Article
Lin, HY; Wang, XF; Ng-Eaton, E; Weinberg, RA; Lodish, HF
Published in: Cell
February 21, 1992

A cDNA encoding the TGF-beta type II receptor protein has been isolated by an expression cloning strategy. The cloned cDNA, when transfected into COS cells, leads to overexpression of an approximately 80 kd protein that specifically binds radioiodinated TGF-beta 1. Excess TGF-beta 1 competes for binding of radioiodinated TGF-beta 1 in a dose-dependent manner and is more effective than TGF-beta 2. The predicted receptor structure includes a cysteine-rich extracellular domain, a single hydrophobic transmembrane domain, and a predicted cytoplasmic serine/threonine kinase domain. A chimeric protein containing the intracellular domain of the type II receptor and expressed in E. coli can phosphorylate itself on serine and threonine residues in vitro, indicating that the cytoplasmic domain of the type II receptor is a functional kinase. This result implicates serine/threonine phosphorylation as an important mechanism of TGF-beta receptor-mediated signaling.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

February 21, 1992

Volume

68

Issue

4

Start / End Page

775 / 785

Location

United States

Related Subject Headings

  • Threonine
  • Serine
  • Sequence Alignment
  • Receptors, Transforming Growth Factor beta
  • Receptors, Cell Surface
  • Protein Kinases
  • Phosphorylation
  • Open Reading Frames
  • Molecular Sequence Data
  • Membrane Proteins
 

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Lin, H. Y., Wang, X. F., Ng-Eaton, E., Weinberg, R. A., & Lodish, H. F. (1992). Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase. Cell, 68(4), 775–785. https://doi.org/10.1016/0092-8674(92)90152-3
Lin, H. Y., X. F. Wang, E. Ng-Eaton, R. A. Weinberg, and H. F. Lodish. “Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase.Cell 68, no. 4 (February 21, 1992): 775–85. https://doi.org/10.1016/0092-8674(92)90152-3.
Lin HY, Wang XF, Ng-Eaton E, Weinberg RA, Lodish HF. Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase. Cell. 1992 Feb 21;68(4):775–85.
Lin, H. Y., et al. “Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase.Cell, vol. 68, no. 4, Feb. 1992, pp. 775–85. Pubmed, doi:10.1016/0092-8674(92)90152-3.
Lin HY, Wang XF, Ng-Eaton E, Weinberg RA, Lodish HF. Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase. Cell. 1992 Feb 21;68(4):775–785.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

February 21, 1992

Volume

68

Issue

4

Start / End Page

775 / 785

Location

United States

Related Subject Headings

  • Threonine
  • Serine
  • Sequence Alignment
  • Receptors, Transforming Growth Factor beta
  • Receptors, Cell Surface
  • Protein Kinases
  • Phosphorylation
  • Open Reading Frames
  • Molecular Sequence Data
  • Membrane Proteins