In vivo functional analysis of in vitro protein binding sites in the immunoglobulin heavy chain enhancer.

Published

Journal Article

We have systematically investigated the functional role of protein binding sites within the mouse immunoglobulin heavy chain enhancer which we previously identified by in vitro binding studies (1,2). Each binding site was deleted, mutant enhancers were cloned 3' of the chloramphenicol acetyl transferase gene in the vector pA10CAT2 and transfected into plasmacytoma cells. We demonstrate that the newly identified site E, located at 324-338 bp, is important for enhancer function; previously identified sites B(uE1), Cl(uE2), C2(uE3) and C3 were also shown to be important for enhancer activity. Sites A and D are not required for IgH enhancer function, as assayed by our methods. Thus, including the octamer site, six protein binding sites which bind at least six different proteins are important for enhancer function in vivo.

Full Text

Duke Authors

Cited Authors

  • Tsao, BP; Wang, XF; Peterson, CL; Calame, K

Published Date

  • April 25, 1988

Published In

Volume / Issue

  • 16 / 8

Start / End Page

  • 3239 - 3253

PubMed ID

  • 3131736

Pubmed Central ID

  • 3131736

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/16.8.3239

Language

  • eng

Conference Location

  • England