Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy.
Journal Article (Letter)
Quantitative phase microscopy is applied to image temporal changes in the refractive index (RI) distributions of solutions created by microbicidal films undergoing hydration. We present a novel method of using an engineered polydimethylsiloxane structure as a static phase reference to facilitate calibration of the absolute RI across the entire field. We present a study of dynamic structural changes in microbicidal films during hydration and subsequent dissolution. With assumptions about the smoothness of the phase changes induced by these films, we calculate absolute changes in the percentage of film in regions across the field of view.
Full Text
Duke Authors
Cited Authors
- Rinehart, MT; Drake, TK; Robles, FE; Rohan, LC; Katz, D; Wax, A
Published Date
- December 2011
Published In
Volume / Issue
- 16 / 12
Start / End Page
- 120510 -
PubMed ID
- 22191912
Pubmed Central ID
- PMC3253752
Electronic International Standard Serial Number (EISSN)
- 1560-2281
International Standard Serial Number (ISSN)
- 1083-3668
Digital Object Identifier (DOI)
- 10.1117/1.3665439
Language
- eng