Measuring cellular structure at submicrometer scale with light scattering spectroscopy


Journal Article

We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells.

Full Text

Duke Authors

Cited Authors

  • Backman, V; Gopal, V; Kalashnikov, M; Badizadegan, K; Gurjar, R; Wax, A; Georgakoudi, I; Mueller, M; Boone, CW; Dasari, RR; Feld, MS

Published Date

  • November 1, 2001

Published In

Volume / Issue

  • 7 / 6

Start / End Page

  • 887 - 893

International Standard Serial Number (ISSN)

  • 1077-260X

Digital Object Identifier (DOI)

  • 10.1109/2944.983289

Citation Source

  • Scopus