1α,25 dihydroxyvitamin D3and mononuclear phagocytes: Enhancement of mouse macrophage and human monocyte hydrogen peroxide production without alteration of tumor cytolysis
1α,25 dihydroxyvitamin D3(1,25 D3) is known to interact with hematopoietic cells. The purpose of this study was to determine the effect of 1,25 D3on hydrogen peroxide (H2O2) production and tumor cell killing by mouse peritoneal macrophages and human blood monocytes. Enhanced monocyte and macrophage phorbol myristate acetate (PMA)-stimulated H2O2production was observed at concentrations of 0.13 to 130 nM 1,25 D3and was maximal at 1.3 nM. At concentrations of 100 U/ml, gamma interferon (IFN-γ) alone had a similar effect but, in combination with 1,25 D3, there was no cooperative effect. At concentrations ranging from 0.13 to 130 nM, 1,25 D3failed to augment tumor cell lysis by macrophages from peptone-injected normal or bacillus Calmette-Guerin (BCG)-infected mice, or by blood monocytes from normal humans. Our results indicate that 1,25 D3can activate the monocyte and macrophage for H2O2secretion without concomitant activation for tumor cell killing.
Volume / Issue
Start / End Page
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)