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Dopamine inhibits burst-firing of neurosecretory cell R 15 in Aplysia californica: establishment of a dose-response relationship.

Publication ,  Journal Article
Gospe, SM; Wilson, WA
Published in: J Pharmacol Exp Ther
July 1980

Burst-firing in neurosecretory cell R 15 of Aplysia californica is inhibited by the bath application of dopamine. Voltage-clamp analysis reveals a complex series of changes in the current-voltage relationship of R 15. Dopamine reduces the inward current in the region of negative slope conductance and tends to linearize the current-voltage curve. A variable increase in potassium conductance is frequently observed. The loss of inward current in the region of negative slope conductance is dose-dependent, highly reproducible and relatively resistant to desensitization, and for these reasons a valid dose-response relationship can be described. The dose-response curve is sigmoidal in shape and rises from a minimal effect near 50 microM to a maximal effect near 500 microM. This dose-response curve is useful as a standard of comparison in studies of agonists and antagonists. A protocol for the quantitative study of this system is described, and the constellation of changes in the current-voltage curve produced by dopamine is discussed.

Duke Scholars

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

July 1980

Volume

214

Issue

1

Start / End Page

112 / 118

Location

United States

Related Subject Headings

  • Pharmacology & Pharmacy
  • Pargyline
  • Neurons
  • Imipramine
  • Dose-Response Relationship, Drug
  • Dopamine
  • Cell Membrane
  • Aplysia
  • Animals
  • Action Potentials
 

Citation

APA
Chicago
ICMJE
MLA
NLM

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

July 1980

Volume

214

Issue

1

Start / End Page

112 / 118

Location

United States

Related Subject Headings

  • Pharmacology & Pharmacy
  • Pargyline
  • Neurons
  • Imipramine
  • Dose-Response Relationship, Drug
  • Dopamine
  • Cell Membrane
  • Aplysia
  • Animals
  • Action Potentials