Dopamine inhibits burst-firing of neurosecretory cell R 15 in Aplysia californica: establishment of a dose-response relationship.
Burst-firing in neurosecretory cell R 15 of Aplysia californica is inhibited by the bath application of dopamine. Voltage-clamp analysis reveals a complex series of changes in the current-voltage relationship of R 15. Dopamine reduces the inward current in the region of negative slope conductance and tends to linearize the current-voltage curve. A variable increase in potassium conductance is frequently observed. The loss of inward current in the region of negative slope conductance is dose-dependent, highly reproducible and relatively resistant to desensitization, and for these reasons a valid dose-response relationship can be described. The dose-response curve is sigmoidal in shape and rises from a minimal effect near 50 microM to a maximal effect near 500 microM. This dose-response curve is useful as a standard of comparison in studies of agonists and antagonists. A protocol for the quantitative study of this system is described, and the constellation of changes in the current-voltage curve produced by dopamine is discussed.
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Related Subject Headings
- Pharmacology & Pharmacy
- Pargyline
- Neurons
- Imipramine
- Dose-Response Relationship, Drug
- Dopamine
- Cell Membrane
- Aplysia
- Animals
- Action Potentials
Citation
Published In
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Pharmacology & Pharmacy
- Pargyline
- Neurons
- Imipramine
- Dose-Response Relationship, Drug
- Dopamine
- Cell Membrane
- Aplysia
- Animals
- Action Potentials