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Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution.

Publication ,  Journal Article
Zalutsky, MR; Xu, FJ; Yu, Y; Foulon, CF; Zhao, XG; Slade, SK; Affleck, DJ; Bast, RC
Published in: Nucl Med Biol
October 1999

Monoclonal antibody (MAb) internalization can have a major effect on tumor retention of radiolabel. Two anti-HER-2/neu MAbs (TA1 and 520C9) were radioiodinated using the iodogen, N-succinimidyl 5-iodo-3-pyridinecarboxylate (SIPC), and tyramine-cellobiose (TCB) methods. Paired-label studies compared internalization and cellular processing of the labeled MAbs by SKOv3 9002-18 ovarian cancer cells in vitro. Intracellular radioiodine activity for 520C9 was up to 2.6 and 3.0 times higher for SIPC and TCB labeling, respectively, compared with iodogen. Likewise, intracellular activity for TA1 was up to 2.3 and 2.9 times higher with the SIPC and TCB methods compared with iodogen labeling. Unfortunately, similar advantages in tumor accumulation were not achieved in athymic mice bearing SKOv3 9008-18 ovarian cancer xenografts.

Duke Scholars

Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

October 1999

Volume

26

Issue

7

Start / End Page

781 / 790

Location

United States

Related Subject Headings

  • Urea
  • Tyramine
  • Tumor Cells, Cultured
  • Tissue Distribution
  • Succinimides
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Ovarian Neoplasms
  • Nuclear Medicine & Medical Imaging
  • Nicotinic Acids
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zalutsky, M. R., Xu, F. J., Yu, Y., Foulon, C. F., Zhao, X. G., Slade, S. K., … Bast, R. C. (1999). Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution. Nucl Med Biol, 26(7), 781–790. https://doi.org/10.1016/s0969-8051(99)00060-8
Zalutsky, M. R., F. J. Xu, Y. Yu, C. F. Foulon, X. G. Zhao, S. K. Slade, D. J. Affleck, and R. C. Bast. “Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution.Nucl Med Biol 26, no. 7 (October 1999): 781–90. https://doi.org/10.1016/s0969-8051(99)00060-8.
Zalutsky MR, Xu FJ, Yu Y, Foulon CF, Zhao XG, Slade SK, et al. Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution. Nucl Med Biol. 1999 Oct;26(7):781–90.
Zalutsky, M. R., et al. “Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution.Nucl Med Biol, vol. 26, no. 7, Oct. 1999, pp. 781–90. Pubmed, doi:10.1016/s0969-8051(99)00060-8.
Zalutsky MR, Xu FJ, Yu Y, Foulon CF, Zhao XG, Slade SK, Affleck DJ, Bast RC. Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution. Nucl Med Biol. 1999 Oct;26(7):781–790.
Journal cover image

Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

October 1999

Volume

26

Issue

7

Start / End Page

781 / 790

Location

United States

Related Subject Headings

  • Urea
  • Tyramine
  • Tumor Cells, Cultured
  • Tissue Distribution
  • Succinimides
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Ovarian Neoplasms
  • Nuclear Medicine & Medical Imaging
  • Nicotinic Acids