Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling.
Journal Article (Journal Article)
Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.
Full Text
Duke Authors
Cited Authors
- Olafsen, T; Bruland, OS; Zalutsky, MR; Sandlie, I
Published Date
- August 1995
Published In
Volume / Issue
- 22 / 6
Start / End Page
- 765 - 771
PubMed ID
- 8535337
International Standard Serial Number (ISSN)
- 0969-8051
Digital Object Identifier (DOI)
- 10.1016/0969-8051(95)00014-o
Language
- eng
Conference Location
- United States