Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling.

Journal Article (Journal Article)

Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.

Full Text

Duke Authors

Cited Authors

  • Olafsen, T; Bruland, OS; Zalutsky, MR; Sandlie, I

Published Date

  • August 1995

Published In

Volume / Issue

  • 22 / 6

Start / End Page

  • 765 - 771

PubMed ID

  • 8535337

International Standard Serial Number (ISSN)

  • 0969-8051

Digital Object Identifier (DOI)

  • 10.1016/0969-8051(95)00014-o


  • eng

Conference Location

  • United States