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Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability.

Publication ,  Journal Article
Garg, PK; Slade, SK; Harrison, CL; Zalutsky, MR
Published in: Int J Rad Appl Instrum B
1989

The effect of para vs meta substitution on the biological behavior of an intact antibody and an F(ab')2 fragment was investigated. Paired-label studies were performed using 81C6 IgG and OC 125 F(ab')2 labeled using the N-succinimidyl esters of both p-[125I]- and m-[131I]iodobenzoate as well as with the potential catabolites, p-[125I]- and m-[131I]iodobenzoic acid. In all 3 studies, up to 55% lower uptake of 131I in thyroid and stomach was observed, suggesting that the m-substituted species were more inert to dehalogenation in vivo.

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Published In

Int J Rad Appl Instrum B

DOI

ISSN

0883-2897

Publication Date

1989

Volume

16

Issue

7

Start / End Page

669 / 673

Location

England

Related Subject Headings

  • Tissue Distribution
  • Neoplasm Proteins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Isotope Labeling
  • Iodobenzoates
  • Iodine Radioisotopes
  • Immunoglobulin Fab Fragments
  • Drug Stability
 

Citation

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Garg, P. K., Slade, S. K., Harrison, C. L., & Zalutsky, M. R. (1989). Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. Int J Rad Appl Instrum B, 16(7), 669–673. https://doi.org/10.1016/0883-2897(89)90136-0
Garg, P. K., S. K. Slade, C. L. Harrison, and M. R. Zalutsky. “Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability.Int J Rad Appl Instrum B 16, no. 7 (1989): 669–73. https://doi.org/10.1016/0883-2897(89)90136-0.
Garg PK, Slade SK, Harrison CL, Zalutsky MR. Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. Int J Rad Appl Instrum B. 1989;16(7):669–73.
Garg, P. K., et al. “Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability.Int J Rad Appl Instrum B, vol. 16, no. 7, 1989, pp. 669–73. Pubmed, doi:10.1016/0883-2897(89)90136-0.
Garg PK, Slade SK, Harrison CL, Zalutsky MR. Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. Int J Rad Appl Instrum B. 1989;16(7):669–673.

Published In

Int J Rad Appl Instrum B

DOI

ISSN

0883-2897

Publication Date

1989

Volume

16

Issue

7

Start / End Page

669 / 673

Location

England

Related Subject Headings

  • Tissue Distribution
  • Neoplasm Proteins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Isotope Labeling
  • Iodobenzoates
  • Iodine Radioisotopes
  • Immunoglobulin Fab Fragments
  • Drug Stability