Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability.

Published

Journal Article

The effect of para vs meta substitution on the biological behavior of an intact antibody and an F(ab')2 fragment was investigated. Paired-label studies were performed using 81C6 IgG and OC 125 F(ab')2 labeled using the N-succinimidyl esters of both p-[125I]- and m-[131I]iodobenzoate as well as with the potential catabolites, p-[125I]- and m-[131I]iodobenzoic acid. In all 3 studies, up to 55% lower uptake of 131I in thyroid and stomach was observed, suggesting that the m-substituted species were more inert to dehalogenation in vivo.

Full Text

Duke Authors

Cited Authors

  • Garg, PK; Slade, SK; Harrison, CL; Zalutsky, MR

Published Date

  • 1989

Published In

Volume / Issue

  • 16 / 7

Start / End Page

  • 669 - 673

PubMed ID

  • 2613522

Pubmed Central ID

  • 2613522

International Standard Serial Number (ISSN)

  • 0883-2897

Language

  • eng

Conference Location

  • England